{ "schemaVersion": 1, "analysisVersion": "s5-projected-length-1", "source": { "pmcid": "PMC4889376", "movie": "S5", "originalUrl": "https://static-movie-usa.glencoesoftware.com/source/10.1073/954/83d8dfe5ff4b12329308937ddfc5a953d6932ec4/pnas.1524807113.sm05.mov", "sha256": "6505eccf821ac1046fab6cf7ab8780df84c502bc738445400b72db5efd64e58b", "widthPx": 5, "heightPx": 132, "frames": 70, "fieldHeightNm": 395.56, "nmPerPixel": 2.9966666666666666, "frameIntervalSeconds": 2, "timingBasis": "Caption 20x speedup × 10 playback fps; nominal, no acquisition timestamps.", "scaleBasis": "Caption field height / 132 raster rows; no instrument calibration uncertainty supplied." }, "method": { "signal": "Maximum red-channel intensity across all five original pixels in each row; no height conversion.", "threshold": 80, "sensitivityThresholds": [ 40, 60, 80, 100, 120 ], "holeClosingPx": 1, "minimumComponentPixels": 12, "anchorRow": 100, "referenceFrame": 12, "referenceSeconds": 24, "referenceReason": "Exclude frames 0–11: isolated/transient objects and a missing segmentation at frame 11. Track continuous component from frame 12.", "uncertainty": "Envelope over thresholds 40–120 plus ±1 pixel per endpoint; reference uncertainty propagated by interval subtraction. Sensitivity bounds, not a 95% CI.", "pauseRule": "Maximal windows >=6 s with nominal projected length range <=2 pixels and common uncertainty interval. Overlapping windows are not independent events.", "review": "Automated digitization with whole-strip visual inspection by Codex; no independent human annotation.", "limitations": [ "Projected end-to-end length, not contour length or AFM height.", "Subtracting both endpoints cancels rigid translation, not rotation, deformation or changing threshold contrast.", "No confirmed zero-growth stops; pause windows can include unresolved slow growth. No population dwell-time distribution.", "Endpoint choice, nonlinear display colors, tip convolution and field calibration have additional unquantified systematic error.", "Model predicts one fast end. Comparing total length assumes a stationary slow end; visible lower-end motion cannot be separated into growth and drift.", "One selected movie from the same publication as calibration inputs; not a held-out biological validation." ] }, "rows": [ { "frame": 0, "timeSeconds": 0, "accepted": false, "reason": "early attachment / identity ambiguous; not scored", "thresholdEdges": [ { "threshold": 40, "endpointsPx": null }, { "threshold": 60, "endpointsPx": null }, { "threshold": 80, "endpointsPx": null }, { "threshold": 100, "endpointsPx": null }, { "threshold": 120, "endpointsPx": null } ], "topPx": null, "bottomPx": null, "lengthPx": null, "lengthNm": null, "lengthBoundsNm": null, "growthNm": null, "growthBoundsNm": null }, { "frame": 1, "timeSeconds": 2, "accepted": false, "reason": "early attachment / identity ambiguous; not scored", "thresholdEdges": [ { "threshold": 40, "endpointsPx": null }, { "threshold": 60, "endpointsPx": null }, { "threshold": 80, "endpointsPx": null }, { 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